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Here, we present a novel framework and experimental method to estimate the mobilization potential of mixed communities. We compare the transfer frequency of a mobilizable plasmid to that of a Here, we present a novel experimental method to estimate the plasmid mobilization potential of a mixed bacterial community, usingIncQRSF1010asmodelplasmid.Wequantifythemobiliza- tion potential of a model community extracted from a domestic shower conduit. Here, we present a novel framework and experimental method to estimate the mobilization potential of mixed communities. We compare the transfer frequency of a mobilizable plasmid to that of a mobilizing and conjugal plasmid measured for a model strain and for the assayed community. Frontiers in Journal Methods 25.11.2014 1 Novel assay to measure the plasmid mobilizing potential of mixed 2 microbial communities 3 4 Uli Klümper1, Ariadni Droumpali1, Arnaud Dechesne1, Barth F Novel assay to measure the plasmid mobilizing potential of mixed microbial communities By Uli Klümper, Ariadni Droumpali, Arnaud Dechesne and Barth F. Smets Download PDF (3 MB) Novel assay to measure the plasmid mobilizing potential of mixed microbial communities By Uli Klümper, Ariadni Droumpali, Arnaud Dechesne and Barth F. Smets Cite Novel assay to measure the plasmid mobilizing potential of mixed microbial communities . By Bahl, Barth, Boe-Hansen, Buchanan-Wollaston, Dechesne, Dubey, Novel assay to measure the plasmid mobilizing potential of mixed microbial communities By Barth Smets , Arnaud Dechesne , and Uli Klümper An individual-based approach to explain plasmid invasion in bacterial populations Here, we present a novel framework and experimental method to estimate the mobilization potential of mixed communities. We compare the transfer frequency of a mobilizable plasmid to that of a mobilizing and conjugal plasmid measured for a model strain and for the assayed community.

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assassination/M assault/MDRGVS assaulter/M assaultive/PY assay/ZRDSG assertional assertive/PY assertiveness/SM assess/ALGSDB assessment/AMS backwoodsmen backyard/SM bacon/SRM baconer/M bacteria/MS bacterial/Y boogieing boohoo/SGD book/JAGZDRMBSU bookbind/JGRZ bookbinder/M  The PLC isoform is sperm-specific and is required for the Ca 2+ mobilization and -, activated by DAG, Ca 2+ and phospholipids), the novel isoforms (PKC, It is possible that orexins increase Ca 2+ influx in the presynaptic part and measure PLC activity, a binding-protein IP 3 assay (biochemical method) was also used. assaulting. assaults.

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Together they form a unique fingerprint. The emergence of m obile c olistin r esistance (mcr) threatens to undermine the clinical efficacy of the last antibiotic that can be used to treat serious infections caused by Gram-negative pathogens.Here we measure the fitness cost of a newly discovered MCR-3 using in vitro growth and competition assays. mcr-3 expression confers a lower fitness cost than mcr-1, as determined by competitive Methods for the robust quantification of bacterial communities are still under development. In this context, the present study aimed to evaluate a method combining competitive PCR (cPCR) and microarray assays for the determination of absolute content of total bacteria and individual bacterial species in samples.

Authors: Uli Klümper, Ariadni Droumpali, Arnaud Dechesne, Barth F. Smets Abstract: Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. Uli Klümper; Ariadni Droumpali; Arnaud Dechesne; Barth F. Smets; Frontiers in Microbiology. Published on 21 Dec 2014. 8,751 views XX downloads; XX citations; View Antibiotic resistance gene transfer mediated by plasmids is a matter of concern for public health, but permissive environments supporting plasmid dissemination are still quite difficult to identify. Lately, we have reported a molecular approach based on quantitative PCR (qPCR) to monitor the fate of the IncP-1β plasmid pB10 in natural microbial communities maintained in microcosms. 2017-07-12 2014-02-10 Novel assay to measure the plasmid mobilizing potential of mixed microbial communities.
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Lanes: 0, molecular size ladder; 1, Agrobacterium 1998-12-01 2020-10-26 The ability to introduce individual molecules of plasmid DNA into cells by transformation has been of central importance to the recent rapid advancement of plasmid biology and to the development of DNA cloning methods.

Here, we present a novel framework and experimental method to estimate the mobilization potential of mixed communities. We compare the transfer frequency of a mobilizable plasmid to that of a mobilizing and conjugal plasmid measured for a model strain and for the assayed community. Frontiers in Journal Methods 25.11.2014 1 Novel assay to measure the plasmid mobilizing potential of mixed 2 microbial communities 3 4 Uli Klümper1, Ariadni Droumpali1, Arnaud Dechesne1, Barth F We thank L. Riber and S. J. Sørensen for access to the tagged RSF1010 plasmid, L. K. Jensen for technical assistance in the laboratory and S. M. Milani for assistance in FACS sorting. This work was funded by the Villum Kann Rasmussen Foundation Center of Excellence CREAM (Center for Environmental and Agricultural Microbiology).
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2021-01-08 1998-03-01 2016-09-23 2018-11-23 Moreover, conjugation via plasmid transfer is postulated to be prevalent in complex microbial communities (e.g., the gut and soil microbiomes) due to the high local density, diversity, and abundance of strains/species along with mobile genetic elements (Ogilvie et al, 2012). 2021-03-25 Dive into the research topics where Ariadni Droumpali is active. These topic labels come from the works of this person.

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By Bahl, Barth, Boe-Hansen, Buchanan-Wollaston, Dechesne, Dubey, Novel assay to measure the plasmid mobilizing potential of mixed microbial communities By Barth Smets , Arnaud Dechesne , and Uli Klümper An individual-based approach to explain plasmid invasion in bacterial populations Here, we present a novel framework and experimental method to estimate the mobilization potential of mixed communities. We compare the transfer frequency of a mobilizable plasmid to that of a mobilizing and conjugal plasmid measured for a model strain and for the assayed community. Novel assay to measure the plasmid mobilizing potential of mixed microbial communities. Is Necessary for the Active Form of ParA From Myxococcus pMF1 Plasmid.

2014;5:730. pmid:25566238 .